Enzymes and Inhibitors
Agilent Technologies N-GLYCANASE PLUS 400MU
PNGase F, peptide-N-glycosidase F,peptide-N4-(N-acetyl--glucosaminyl)asparagine amidase.Releases intact N-glycans by cleavingbetween the innermost GlcNAc and Asn.
Carnabio Usa Inc BTN - RET / 10UG
BTN-RET, Cytoplasmic domain, Wild type, Amino Acid 658-1114(end), NP_066124.1, 56 kDa, Insect (sf21); 10microg
Echelon Biosciences Research Labs FS-3 (LysoPLD / Autotaxin substrate) 100 ug
Echelons fluorogenic Autotaxin (ATX) substrate FS-3 (L-2000) is a doubly labeled analog of LPC wherin the fluorophore is quenched through intramolecular energy transfer. Hydrolysis of the substrate produces an increase in fluorescence. This item is non returnable
Carnabio Usa Inc BTN - LCK / 10UG
BTN-LCK, Full-length, Wild type, Amino Acid 1-509(end), NP_005347.2, 62 kDa, Insect (sf21); 10microg
Supply Solutions Roche - Proteinase K,Recomb.,Pcr Grd. Lyo.,25 Mg
Roche - Proteinase K,recomb.,PCR Grd. lyo.,25 mg; Lyophilized from Pichia pastoris; pkg of 100 mg (03115879001) pkg of 25 mg (03115836001) pkg of 2 x 250 mg (03115801001) pkg of 4 x 250 mg (0311582001)
Supply Solutions Roche - Expand HiFi PCR Sys., 500 u; pkg of 2,500 U (11759078001 [10x250 U]) pkg of 100 U (11732641001) pkg of 500 U (11732650001 [2x250 U])
Roche - Expand HiFi PCR Sys., 500 u; pkg of 2,500 U (11759078001 [10x250 U]) pkg of 100 U (11732641001) pkg of 500 U (11732650001 [2x250 U])
Supply Solutions Roche - RNase A, 100 mg; from bovine pancreas; pkg of 100 mg (10109169001) pkg of 25 mg (10109142001)
Roche - RNase A, 100 mg; from bovine pancreas; pkg of 100 mg (10109169001) pkg of 25 mg (10109142001)
INTACT GENOMICS INC PEmax Enzyme 80 µg
Intact Genomics (IG) PEmax enzyme is the purified recombinant Streptococcus pyogenes Cas9 nickase mutant (H840A)-Murine Moloney Leukemia Virus-Reverse Transcriptase (MMLV-RT) fusion protein3-4 containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR prime-editing3-4. The physical purity of this enzyme is ≥85% as assessed by SDS-PAGE with Coomassie blue staining.Product Includes: PEmax enzyme 5x PEmax Nicking Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM MgCl2, 0.1 mM EDTA, pH 6.5 @ 25 °CStore all components at -20°C.
INTACT GENOMICS INC RNase Inhibitor, Murine 20,000 Units
RNase inhibitors are commonly used to inhibit and control RNase contaminants in enzymatic manipulations of RNA. Intact Genomics RNase Inhibitor is a 49.8 KDa recombinant protein of murine origin which specifically inhibits RNases A, B and C by binding noncovalently in a 1:1 ratio with high affinity. Moreover, it does not inhibit polymerase activity when used with Taq DNA Polymerase, Reverse Transcriptase. This Inhibitor from Murine is resistance to oxidation compared to the human/porcine inhibitors as it lacks the pair of cysteines found in the human/porcine versions. In addition, it is stable at less than 1 mM DTT concentration.Applications cDNA synthesis RT-PCR/RT-qPCR In vitro transcription/translation RNA labeling
New England Biolabs, Inc. HphI – 1000 units
A restriction endonuclease that recognizes the sequence GGTGANNNNNNN_N^.
New England Biolabs, Inc. T4 RNA Ligase 2 – 150 units
T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity). Unlike T4 RNA Ligase 1 , T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA. The enzyme requires an adjacent 5 phosphate and 3' OH for ligation. The enzyme can also ligate the 3' OH of RNA to the 5 phosphate of DNA in a double stranded structure.
New England Biolabs, Inc. NgoMIV – 1000 units
A restriction endonuclease that recognizes the sequence G^CCGG_C.